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1994-09-19
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Document 0409
DOCN M9490409
TI The core and carboxyl-terminal domains of the integrase protein of human
immunodeficiency virus type 1 each contribute to nonspecific DNA
binding.
DT 9411
AU Engelman A; Hickman AB; Craigie R; Laboratory of Molecular Biology,
National Institute of Diabetes; and Digestive and Kidney Diseases,
National Institutes of Health,; Bethesda, MD 20892.
SO J Virol. 1994 Sep;68(9):5911-7. Unique Identifier : AIDSLINE
MED/94335108
AB The integrase protein of human immunodeficiency virus type 1 removes two
nucleotides from the 3' ends of reverse-transcribed human
immunodeficiency virus type 1 DNA (3' processing) and covalently inserts
the processed ends into a target DNA (DNA strand transfer). Mutant
integrase proteins that lack the amino-and/or carboxyl-terminal domains
are incapable of catalyzing 3' processing and DNA strand transfer but
are competent for an apparent reversal of the DNA strand transfer
reaction (disintegration) in vitro. Here, we investigate the binding of
integrase to DNA by UV cross-linking. Cross-linked complexes form with a
variety of DNA substrates independent of the presence of divalent metal
ion. Analysis with amino- and carboxyl-terminal deletion mutant proteins
shows that residues 213 to 266 of the 288-residue protein are required
for efficient cross-linking in the absence of divalent metal ion.
Carboxyl-terminal deletion mutants that lack this region efficiently
cross-link only to the branched disintegration DNA substrate, and this
reaction is dependent on the presence of metal ion. Both the core and
C-terminal domains of integrase therefore contribute to nonspecific DNA
binding.
DE DNA Nucleotidyltransferases/*METABOLISM DNA-Binding
Proteins/*METABOLISM DNA, Single-Stranded/METABOLISM DNA,
Viral/METABOLISM HIV-1/*ENZYMOLOGY Photochemistry Recombinant
Proteins Sequence Deletion Structure-Activity Relationship Support,
U.S. Gov't, P.H.S. Ultraviolet Rays *Virus Integration JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).